Role of Nrf2 in Lipopolysaccharide-Induced Acute Kidney Injury: Protection by Human Umbilical Cord Blood Mononuclear Cells

Figure 7

Ultrastructural changes and the changes of mitochondrial damage-associated proteins and autophagy-associated proteins of cells. Western blot was used to detect the expression of mfn2, Drp1, p62, PINK1, Parkin, and LC3-II. Mitochondria injury in rat kidney tissues was observed by transmission electron microscopy (TEM). (a) Representative blots of mfn2, Drp1, p62, PINK1, Parkin, LC3-II, and GAPDH. (b) Densitometry of mfn2. (c) Densitometry of Drp1. (d) Densitometry of p62. (e) Densitometry of PINK1. (f) Densitometry of Parkin. (g) Densitometry of LC3-II. (h) Representative photomicrographs of cells. Typical autophagosome (red arrow) was observed in the LPS+TBHQ group (original magnifications: ×10000, under a Hitachi H7700 electron microscope). Values are , for each group. ^★ versus the control group. ^# versus the LPS group. ^∆ versus the LPS+TBHQ group.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)