Mild Oxidative Stress Reduces NRF2 SUMOylation to Promote Kras/Lkb1/Keap1 Mutant Lung Adenocarcinoma Cell Migration and Invasion

Figure 4

Mild ROS act as signaling molecules to activate the JNK/c-Jun axis and promote KLK LUAD cell migration and invasion. (a) The altered activation of MAPK signaling pathways (ERK, JNK, and p38 signaling pathways) in four stable cell lines constructed from A549 cells. MAPK: mitogen-activated protein kinase; ERK: extracellular signal-regulated kinase; JNK: c-Jun NH-2 terminal kinase. Blots were quantified and normalized to β-actin expression. (b) Heat map comparing patterns of gene expression related to cell and cell adhesion as well as focal adhesion in four stable cell lines constructed from A549 cells. (c) Trend graph comparing patterns of gene expression related to cell and cell adhesion as well as focal adhesion in four stable cell lines constructed from A549 cells. (d) Validation of expression of genes involved in cell and cell adhesion as well as focal adhesion by quantitative real-time PCR in four stable cell lines constructed from A549 cells (3 replicates per group). Podxl: podocalyxin like; Cdh4: cadherin 4; Mcam: melanoma cell adhesion molecule; Cav1: caveolin 1; Itgb3: integrin subunit beta 3; Tgfa: transforming growth factor alpha; Loxl2: lysyl oxidase like 2; Ajuba: Ajuba LIM protein. , , and .

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