miR-155-5p Implicates in the Pathogenesis of Renal Fibrosis via Targeting SOCS1 and SOCS6

Figure 1

miR-155-5p is highly expressed in the fibrotic kidney tissues. (a) RT-qPCR showed the expression level of miR-155-5p in the kidney tissues from normal donors (ND, ) and tubulointerstitial fibrosis (TIF) patients (). by -test. (b) Immunoblot determined the protein levels of fibronectin and α-SMA in the kidney tissues. UUO: unilateral ureteral obstruction surgery; Mr: molecular weight; KDa: kilo Dalton. (c) RT-qPCR determined miR-155-5p expression in the kidney from UUO rats at different time points (after UUO surgery). , by t-test. (d) Histological analysis determined renal fibrosis injury (H&E staining), α-SMA (IHC), and miR-155-5p (ISH) expression in kidney sections. The optical density value of IHC and ISH was obtained using the ImageJ software, and all the values were normalized by the control group. , by one-way ANOVA. (e) The correlation between miR-155-5p expression and renal injury score/α-SMA expression in UUO rats. R: Pearson’s correlation coefficient, .

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