miR-155-5p Implicates in the Pathogenesis of Renal Fibrosis via Targeting SOCS1 and SOCS6

Figure 3

SOCS1/6 is the direct target of miR-155-5p. (a) SOCS1/5/6 are potential miR-155-5p targets predicted by in-house bioinformatics tools MicroCible and MicroTopTable. (b) Luciferase reporter assay determined the direct binding between SOCS1/5/6 and miR-155-5p, respectively. ; n.s.: no significance, by -test. (c) RT-qPCR determined the expression of SOCS1/5/6 in miR-155-5p overexpressed HK-2 cells. ; n.s.: no significance, and by -test. (d) RT-qPCR determined the expression of SOCS1/5/6 in miR-155-5p overexpressed NRK-49 cells. ; n.s.: no significance, and by -test. (e) Immunoblot and its relative quantification represent the expression of collagen I and α-SMA in HK-2 and NRK-49 cells. ; and by -test. (f) Immunoblot and its relative quantification represent the expression of SOCS1/6 in human kidney tissues. ; and by -test.

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