Research Article
FNDC5 Attenuates Oxidative Stress and NLRP3 Inflammasome Activation in Vascular Smooth Muscle Cells via Activating the AMPK-SIRT1 Signal Pathway
Figure 2
FNDC5 deficiency deteriorates Ang II-induced oxidative stress and NLRP3 inflammasome activation in aorta of mice. Ang II was infused subcutaneously with a microosmotic pump at 400 ng/Kg/min for 2 weeks. (a) Representative images showing the ROS detected by dihydroethidium (DHE) staining. (b) Bar graph showing the relative fluorescence intensity of DHE. (c) NOX2 protein expression. (d) NLRP3, pro-IL-1β, and IL-1β protein expressions. (e) Phosphorylated AMPKα. (f) SIRT1 protein expression. Values are . vs WT; vs Ctrl. per group.
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