FNDC5 Attenuates Oxidative Stress and NLRP3 Inflammasome Activation in Vascular Smooth Muscle Cells via Activating the AMPK-SIRT1 Signal Pathway

Figure 8

FNDC5 prevents the enhanced oxidative stress and NLRP3 inflammasome activation in Ang II-treated VSMCs of FNDC5^-/- mice. The primary VSMCs of the WT and FNDC5 knockout (KO) mice were treated with PBS or FNDC5 (200 nM) for 2 h followed by PBS or Ang II (100 nM) for 24 h. (a) FNDC5 mRNA and protein expressions. (b) Representative images showing the ROS detected by dihydroethidium (DHE) staining. (c) Bar graph showing the relative fluorescence intensity of DHE. (d) NOX2 protein expression. (e) NLRP3, pro-IL-1β, and IL-1β protein expressions. (f) Phosphorylated AMPKα. (g) SIRT1 activity and SIRT1 protein expression. Values are . vs WT; vs PBS or Ctrl. vs Ang II. per group.

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