Hyperglycemia Induces Endoplasmic Reticulum Stress in Atrial Cardiomyocytes, and Mitofusin-2 Downregulation Prevents Mitochondrial Dysfunction and Subsequent Cell Death

Figure 2

High glucose induces mitochondrial oxidative stress and induced apoptosis. (a) Confocal microscopy detected primary atrial myocytes that stained with DCFH-DA. (b) Quantification of ROS by DCFH-DA intensity in (a). Data represent the ( independent experiments). (c, d) JC-1 staining. The ratio of red/green fluorescence reflects changes in the Δψm of primary atrial myocytes. Data represent the ( independent experiments). Scale bar, 100 μm. (e) Mn-SOD, Bax, and Bcl-2 protein level in atrial myocytes detected by Western blot analysis. (f, g) Quantification of Mn-SOD and Bax/Bcl-2 protein level in (e). Data are , independent experiments, , ^#, ANOVA with Bonferroni posttest.

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