A Novel STAT3-Mediated GATA6 Pathway Contributes to tert-Butylhydroquinone- (tBHQ-) Protected TNFα-Activated Vascular Cell Adhesion Molecule 1 (VCAM-1) in Vascular Endothelium

Figure 4

tBHQ inhibits TNFα-activated GATA6 in vascular endothelial cells. HAEC cells were treated with TNFα (10 ng·mL^-1, 6 h for mRNA and 16 h for protein detection). tBHQ (100 μmol·L^-1) was added 1 h before TNFα treatment. (a) Nuclear protein was exacted after the indicated treatment. Western blotting was performed to detect the expression of NFκB (p65), GATA6, SP-1, c-Fos, and c-Jun in nucleus. (b) Total GATA6 expression. (c) mRNA level of Gata6 was detected. (d) HAEC cells were transfected with si GATA6 or scramble siRNA (si NC) and followed by TNFα treatment for 16 h. Western blotting was performed to detect the intracellular expression of VCAM-1 and GATA6. (e) Immunohistochemisty for vascular endothelium GATA6 expression (). In vitro values are denoted as from three or more independent batches of cells. Each group contains the same amount of solvent. nd: none-detective. indicates statistically significant differences. ns: no significant differences.

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