A Novel STAT3-Mediated GATA6 Pathway Contributes to tert-Butylhydroquinone- (tBHQ-) Protected TNFα-Activated Vascular Cell Adhesion Molecule 1 (VCAM-1) in Vascular Endothelium

Figure 5

MAPK pathway is negatively associated with tBHQ-decreased GATA6. HAEC cells were treated with TNFα (10 ng·mL^-1) for 16 h. tBHQ (100 μmol·L^-1) was added 1 h before TNFα treatment. SB202190 (5 μmol·L^-1) or U46619 (10 nmol·L^-1) was added 1 h before tBHQ treatment. (a) Western blotting was performed to detect the expression of p38 MAPKs. (b, c) Expression of VCAM-1 and GATA6. Each group contains the same amount of solvent. Each test was conducted at least 3 times, and a representative blot was shown. nd: none-detective. and indicate statistically significant differences. ns: no significant differences.

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(b)

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