A Novel STAT3-Mediated GATA6 Pathway Contributes to tert-Butylhydroquinone- (tBHQ-) Protected TNFα-Activated Vascular Cell Adhesion Molecule 1 (VCAM-1) in Vascular Endothelium

Figure 6

GATA6 is regulated by STAT3 phosphorylation. HAEC cells were treated with TNFα (10 ng·mL^-1, 6 h for mRNA and 16 h for protein detection). tBHQ (100 μmol·L^-1) was added 1 h before TNFα treatment. SHP099 (25 μmol·L^-1) or Stattic (25 μmol·L^-1) was added 1 h before TNFα treatment. (a, b) Western blotting was performed to detect the expression of total and nuclear phosphorylated STAT3, respectively. (c) The mRNA level of Gata6. (d) Western blotting was performed to detect the expression of phosphorylated STAT3 and GATA6. (e) The mRNA levels of Gata6 and Vcam-1. (f) Western blotting was performed to detect the expression of VCAM-1, phosphorylated STAT3, and GATA6. (g) HAEC cells were transfected with siGATA6 or scramble siRNA (siNC) and followed by the indicated treatment. VCAM-1, phosphorylated STAT3, and GATA6 expression were detected by Western blotting. All values are denoted as from three or more independent batches of cells. Each group contains the same amount of solvent. nd: none-detective. and indicate statistically significant differences. ns: no significant differences.

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