Allicin Attenuated Advanced Oxidation Protein Product-Induced Oxidative Stress and Mitochondrial Apoptosis in Human Nucleus Pulposus Cells

Figure 4

Allicin treatment inhibited AOPP-induced oxidative stress and mitochondrial dysfunction of human NP cells. (a, b) The human NP cells were pretreated with different concentrations of allicin (0, 5, 10, and 20 μM) for 2 h, before treatment with 400 μg/ml AOPP for 24 h. The intracellular ROS levels of the NP cells for each group were detected by ROS-specific fluorescent probe DHE and measured by subsequent flow cytometry analysis. Representative peak charts of flow cytometry and relative quantitative analysis were shown. (c) The intracellular MDA levels (as a marker of lipid peroxidation) of human NP cells were examined by a commercial kit. (d, e) The mitochondrial membrane potential of human NP cells in each group was examined by JC-1 staining and measured by subsequent flow cytometry analysis. The quantitative analysis of the ratio of red fluorescence ( axis) to green fluorescence ( axis) and representative scatter plots of flow cytometry were shown. Data were represented as . ^# and ^## versus the control group; and versus the AOPP alone treatment group, .

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