Allicin Attenuated Advanced Oxidation Protein Product-Induced Oxidative Stress and Mitochondrial Apoptosis in Human Nucleus Pulposus Cells

Figure 5

Effects of allicin on AOPP-induced MAPK pathway activation. (a) The human NP cells were pretreated with various concentrations of allicin (0, 5, 10, and 20 μM) for 2 h, followed by stimulation with 400 μg/ml AOPP for 24 h. The protein levels of ERK, phosphorylated ERK, p38, phosphorylated p38, JNK, and phosphorylated JNK were determined using Western blotting analysis. (b–d) Immunoblot bands corresponded to (b) p-ERK, (c) p-p38, and (d) p-JNK were quantified by densitometric analysis and normalized to their corresponding total kinase. Data were represented as . ^## versus the control group; and versus the AOPP alone treatment group, .

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