Sustained Release of Melatonin from GelMA Liposomes Reduced Osteoblast Apoptosis and Improved Implant Osseointegration in Osteoporosis

Figure 6

Melatonin inhibits osteoblast apoptosis by activating the sirt3 pathway and promotes osteogenic differentiation. (a, b) TUNEL staining and quantitative analysis, with yellow arrows indicating apoptotic cells. per group, , , vs. the GelMA-DOPA@MT group. (c) Cell lysate was subjected to Western blotting with antibodies against the apoptosis-specific proteins Bax and Bcl-2. (d–f) Quantitative analysis of Bax and Bcl-2 protein levels, per group, , , vs. the GelMA-DOPA@MT group. (g–i) qPCR analysis of the expression of the apoptosis-specific genes Bax and Bcl-2 in the experimental group, per group, , , vs. the GelMA-DOPA@MT group.

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