Sustained Release of Melatonin from GelMA Liposomes Reduced Osteoblast Apoptosis and Improved Implant Osseointegration in Osteoporosis

Figure 7

Melatonin promotes osteogenic differentiation by activating the sirt3 pathway. (a) Representative images showing cellular ARS and ALP staining. (b, c) Quantitative analysis of the results of ARS and ALP staining. per group, , , vs. the GelMA-DOPA@MT group. (d) Cell lysate was subjected to Western blotting with antibodies against the osteogenesis-specific proteins ALP and Osterix. (e, f) Quantitative analysis of ALP and Osterix protein levels, per group, , , vs. the GelMA-DOPA@MT group. (g, h) qPCR analysis of the expression of the osteogenesis-specific genes ALP and Osterix in the experimental group, per group, , , vs. the GelMA-DOPA@MT group.

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