Mitochondrial and Peroxisomal Alterations Contribute to Energy Dysmetabolism in Riboflavin Transporter Deficiency

Figure 9

JC-1 staining images of Ctrl iPSCs and RTD iPSCs before and after RF treatment, showing the same field captured before and after 1, 5, 10, 15, and 30 min H₂O₂ exposure. (a) Regions of high mitochondrial polarization are revealed by red fluorescence due to J-aggregate formation, whereas depolarized regions are marked by green fluorescence of JC-1 monomers. Images show the time-dependent loss of red J-aggregate fluorescence and cytoplasmic diffusion of green monomer fluorescence following H₂O₂ administration. (b) Time course of cells following H₂O₂ exposure showing the fluorescence intensity ratio for JC-1 staining (red/green).

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