Research Article

Mitochondrial and Peroxisomal Alterations Contribute to Energy Dysmetabolism in Riboflavin Transporter Deficiency

Figure 9

JC-1 staining images of Ctrl iPSCs and RTD iPSCs before and after RF treatment, showing the same field captured before and after 1, 5, 10, 15, and 30 min H2O2 exposure. (a) Regions of high mitochondrial polarization are revealed by red fluorescence due to J-aggregate formation, whereas depolarized regions are marked by green fluorescence of JC-1 monomers. Images show the time-dependent loss of red J-aggregate fluorescence and cytoplasmic diffusion of green monomer fluorescence following H2O2 administration. (b) Time course of cells following H2O2 exposure showing the fluorescence intensity ratio for JC-1 staining (red/green).