Research Article
Hydrogen Gas Attenuates Hypoxic-Ischemic Brain Injury via Regulation of the MAPK/HO-1/PGC-1a Pathway in Neonatal Rats
Figure 5
H2 inhibited HMGB1/TLR-4 expression in the hippocampal CA3 region of neonatal HIBI rats. (a) The representative images of TLR-4 (red) and glial fibrillary acidic protein (GFAP, green) and 4,6-diamidino-2-phenylindole (DAPI, blue) immunofluorescence staining as well as merged immunofluorescent signals of all markers in the hippocampal CA3 region of sham surgery, HIBI, and H2-90 min groups (scar bar: 50 μm). (b) Representative images of HMGB1 (red) and glial fibrillary acidic protein (GFAP, green) and 4,6-diamidino-2-phenylindole (DAPI, blue) immunofluorescence staining as well as merged immunofluorescent signals of all markers in the hippocampal CA3 region of sham surgery, HIBI, and H2-90 min groups (scale bar: 50 μm). (c) Western blot analysis of TLR-4, HMGB1, and β-actin proteins in the hippocampus of each group. (d, e) Bar graphs of the relative expression of TLR-4, HMGB1 in the hippocampus of each group (; ).
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