Oxidative Medicine and Cellular Longevity

Research Article

Protective Effect of Polydatin on Jejunal Mucosal Integrity, Redox Status, Inflammatory Response, and Mitochondrial Function in Intrauterine Growth-Retarded Weanling Piglets

Table 1

Primer sequences used for real-time PCR assay.


Name¹	GenBank²	Sequence³ (5 → 3)	Length (bp)

ZO1	XM_021098827.1	AGGCGTGTTTAACAGCAACG	182
		CCAAAGGACTCAGCAGGGTT
OCLN	NM_001163647.2	CAGGTGCACCCTCCAGATTG	167
		ATGTCGTTGCTGGGTGCATA
CLDN1	NM_001244539.1	ACAGGAGGGAAGCCATTTTCA	82
		TTTAAGGACCGCCCTCTCCC
CLDN2	NM_001161638.1	GGATCCTGCGGGACTTCTAC	153
		TGGAGCGATTTCCTTGCAGT
CLDN3	NM_001160075.1	GAGACCAGTCCACCCAGATG	147
		AGGTTTCATGGTCCGTGCTG
GAPDH	NM_001206359.1	CCAAGGAGTAAGAGCCCCTG	125
		AAGTCAGGAGATGCTCGGTG
ACTB	XM_003124280.5	TGGAACGGTGAAGGTGACAG	176
		CTTTTGGGAAGGCAGGGACT

¹ACTB: beta actin; CLDN1: claudin 1; CLDN2: claudin 2; CLDN3: claudin 3; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; OCLN: occludin; ZO1: zonula occludens 1. ²GenBank accession number. ³Shown as the forward primer followed by the reverse primer.