Caffeic Acid Phenethyl Ester Protects against Experimental Autoimmune Encephalomyelitis by Regulating T Cell Activities

Figure 3

Effects of CAPE on T cell activation and NF-κB p65 nuclear translocation. Lymphocytes were cultured at a concentration of cells/ml per well in 96-well plates or cells/ml per well in 24-well plates and stimulated with ConA (5 μg/ml). The percentages of CD69 and CD25 in CD4⁺ T cells after ConA stimulation for 8 h or 24 h (a, b). NF-κB p65 nuclear translocation after stimulating splenocytes with ConA for 2 h (c). The data () represent three independent experiments. , , , versus the vehicle group.

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