Notoginseng Leaf Triterpenes Ameliorates OGD/R-Induced Neuronal Injury via SIRT1/2/3-Foxo3a-MnSOD/PGC-1α Signaling Pathways Mediated by the NAMPT-NAD Pathway

Figure 2

Effects of PNGL on cell viability and apoptosis in OGD-induced SH-SY5Y cells. PNGL improved the cell viability and inhibited the apoptosis rate in OGD-induced SH-SY5Y cells, which was partly reversed by the inhibitor FK866. (a) The pretreatment with PNGL (1.56~12.5 μg/mL) for 24 h, followed by OGD/R treatment. (b) The copretreatment of PNGL and FK866 for 24 h, followed by OGD/R treatment. The cell viability was measured by using the MTT assay. (c) The annexin V/propidium iodide (PI) staining in OGD/R-induced SH-SY5Y cells, measured by a flow cytometer. (d) The hoechst33324/PI staining in OGD/R-induced SH-SY5Y cells, measured by a fluorescence microscope. The statistical analysis, analyzed by using the Image J 2.44 software. The data presented as the (). ^#, ^## versus the control group; , versus the OGD/R model group; ^&, ^&& versus the OGD/R+PNGL group. Scale bar, 200 μm.

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