Notoginseng Leaf Triterpenes Ameliorates OGD/R-Induced Neuronal Injury via SIRT1/2/3-Foxo3a-MnSOD/PGC-1α Signaling Pathways Mediated by the NAMPT-NAD Pathway

Figure 4

Effects of PNGL on mitochondria and energy metabolism in OGD-induced SH-SY5Y cells. PNGL improved mitochondria and energy metabolism, partly reversed by FK866, which was partly reversed by the inhibitor FK866. (a, b) The representative images of mitochondria, stained by the Mito-Tracker-CMXRos, were measured by a fluorescence microscope. (c) The statistical data of mitochondria fluorescence value was analyzed by using the ImageJ 2.44 software. (d, e) The ATP and ATPase levels in OGD/R-induced SH-SY5Y cells were detected by ELISA assay kits. (f, g) The NAD and NADH levels were detected by the ELISA assay kits. (h) The ratio of NAD/NADH levels in cells. The data presented as the . ^#, ^## versus the control group; , versus the OGD/R model group; ^&, ^&& versus the OGD/R+PNGL group. Scale bar, 100 μm.

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