Rat Chondrocyte Inflammation and Osteoarthritis Are Ameliorated by Madecassoside

Figure 3

MA upregulates the expression levels of chondrocyte-specific protein. Three groups of MA-pretreated chondrocytes were incubated with and without 10 ng/ml IL-1β, and one group stimulated with IL-1β in the absence of MA for 24 hours. qRT-PCR at the mRNA level for sox-9 and collagen II (a) and (b). Western blotting and quantitative analysis for SOX-9 and COL II in the presence of IL-1β (c), (e), and (f), and the images (d), (g), and (h) in the absence of IL-1β. ; data are expressed as the deviation. vs. sham group. vs. IL-1β group. COL II: collagen II; MA: madecassoside; SOX-9: transcription factor SOX-9; IL: interleukin.