Sirtuin 3 Inhibits Airway Epithelial Mitochondrial Oxidative Stress in Cigarette Smoke-Induced COPD

Figure 2

CSE decreased Sirt3 and MnSOD expression and MnSOD activity in a concentration-dependent manner in airway epithelial cells. When airway epithelial cells were stimulated with CSE (2.5-10%) for 24 h, real-time PCR showed that the mRNA expression of Sirt3 (a) and MnSOD (b) was decreased in a concentration-dependent manner. The protein expression of Sirt3 and MnSOD was determined by Western blot, and a representative blot is shown in part (c). Quantification analysis displayed that the expression levels of Sirt3 (d) and MnSOD (e) were significantly decreased in a concentration-dependent manner when airway epithelial cells were stimulated with CSE (2.5-10%) for 24 h. After 24 h stimulation with CSE, MnSOD activities in airway epithelial cells were gradually decreased at the indicated concentration (f). All statistical data were obtained from three independent experiments and presented as . versus control group.

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