Blockade of Adenosine A_2A Receptor Protects Photoreceptors after Retinal Detachment by Inhibiting Inflammation and Oxidative Stress

Figure 3

ZM241385 markedly reduced microglia proliferation and reactivity in the retina after RD in mice. (a) Retinal sections were stained with antibodies against Iba-1 (red) and CD11b (green). Representative images are depicted, and arrows indicate some Iba-1⁺ and CD11b⁺ cells found in each condition. (b) Retinal sections were stained with antibodies against Iba-1 (red) and MHC-II (green). Representative images are depicted, and arrows indicate some Iba-1⁺ and MHC-II⁺ cells found in each condition. (c) Quantification of Iba-1⁺ cell counts per retinal section in the retina (). (d) Quantification of Iba-1 intensity per mm² in the retina (). (e) Quantification of Iba-1⁺ CD11b⁺ cell counts per retinal section in the retina (). (f) Activated microglia (Iba-1⁺ MHC-II⁺ cells) were counted and normalized to the percentage of total microglial cells (Iba-1⁺ cells) (). , , , , Scale bar: 50 μm.

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