A Newly Synthesized Rhamnoside Derivative Alleviates Alzheimer’s Amyloid-β-Induced Oxidative Stress, Mitochondrial Dysfunction, and Cell Senescence through Upregulating SIRT3

Figure 3

PL171 inhibited Aβ₄₂O-induced reduction of oxygen consumption in SK-N-SH cells. Seahorse assays showing mitochondrial bioenergetics in SK-N-SH cells preincubated without or with PL171 (30 μM) for 4 h followed by stimulation with Aβ₄₂O (10 μM) for 24 h. (a) The representative graph of the mitochondrial stress test detailing the four key parameters of mitochondrial function through sequential addition of oligomycin (1 μM), FCCP (1 μM), and rotenone/antimycin A (1 μM each), which allowed the measurement of basal respiration (b), mitochondrial ATP production (c), and the maximal respiration (d). The data are presented as , independent experiments, , analyzed by one-way ANOVA followed by Bonferroni’s test.

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