Aescin Protects Neuron from Ischemia-Reperfusion Injury via Regulating the PRAS40/mTOR Signaling Pathway

Figure 3

Neuroprotective effects of PRAS40 overexpression after OGD/R in vivo. (a) The effects of PRAS40 overexpression on neuronal death were measured by LDH release as before. Primary cultured neurons were transfected with lentivirus containing PRAS40; controls were cultures transfected with GFP vectors. Then, they were subjected to 2 hours of OGD 96 hours after transfection. LDH release was measured 24 hours post-OGD and reperfusion. All data after OGD was normalized to the values of the sham group. (b) Representative results showed protein bands in the PRAS40/mTOR pathways, including phosphorylated and total protein expression, as measured by western blot. (c) The bar graphs showed the quantified protein levels of pPRAS40, PRAS40, pmTOR, mTOR, pS6K, S6K, p4E-BP1, and 4E-BP1, respectively. All results are given as . vs. sham (no OGD); ^# vs. PRAS40; ^† vs PRAS40. per group. Sham group: no OGD samples; Con group: OGD/R samples without treatment; vector group: OGD/R samples treated with empty vector; PRAS40 KD group: OGD/R samples treated with PRAS40 lentivirus.

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