Downregulated Recycling Process but Not De Novo Synthesis of Glutathione Limits Antioxidant Capacity of Erythrocytes in Hypoxia

Figure 2

Hypoxia limits the antioxidant capacity of RBCs exposed to oxidative stress. Levels of FRAP (a), SOD (b), and CAT (c) in normoxic and hypoxic RBCs after HX/XO exposure (1.5 mM HX; 0.4 U/ml XO; 30 min). Glutathione-related parameters, including GSH (d), TFG (e), and GSH/GSSG ratio (f) in normoxic and hypoxic RBCs. Data represent the of at least three independent experiments. and for normoxic or hypoxic RBCs vs. corresponding RBCs with HX/XO exposure. ^# for normoxic RBCs vs. corresponding hypoxic RBCs.

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