WJ-39, an Aldose Reductase Inhibitor, Ameliorates Renal Lesions in Diabetic Nephropathy by Activating Nrf2 Signaling

Figure 6

WJ-39 alleviated inflammation via the Nrf2 pathway in rat mesangial cells cultured under high glucose (HG) conditions. (a, b) Protein levels of interleukin-1beta (IL-1β), IL-6, IL-18, tumor necrosis factor-alpha (TNF-α), p50 in the nucleus and cytosol, p65 in the nucleus and cytosol, cleaved caspase-1, ASC, and NLRP3 were detected by western blotting and quantified. Data are represented as the (SEM), . ^# and ^## vs. the normal glucose (NG) group; and vs. the HG group; ^& and ^&& vs. the WJ-39 10 μM+HG+control siRNA group. Nrf2: nuclear factor erythroid 2-related factor 2; ASC: apoptosis-associated speck-like protein containing a caspase recruitment domain; NLRP3: nucleotide-binding and oligomerization domain-like receptor family pyrin domain-containing 3.

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