WJ-39, an Aldose Reductase Inhibitor, Ameliorates Renal Lesions in Diabetic Nephropathy by Activating Nrf2 Signaling

Table 1

Primary antibody information for western blotting.

Primary antibodies	Catalog number, supplier
Anti-thioredoxin (TRX)	14999-1-AP, Proteintech, Wuhan, China
Anti-NAD(P)H dehydrogenase [quinone] 1 (NQO1)	ab80588, Abcam, Cambridge, UK
Anti-HO-1	ab68477, Abcam, Cambridge, UK
Anti-AR	15439-1-AP, Proteintech, Wuhan, China
Anti-phosphatidylinositol-3-kinase (PI3K)	#4257, Cell Signaling Technology, Danvers, MA, USA
Anti-p-PI3K	SAB4504314, Sigma-Aldrich, St. Luis, MO, USA
Anti-AKT	sc-81434, Santa Cruz, Dallas, Texas, USA
Anti-p-AKT	sc-514032, Santa Cruz, Dallas, Texas, USA
Anti-Nrf2	ab137550, Abcam, Cambridge, UK
Anti-TGF-β1	ab179695, Abcam, Cambridge, UK
Anti-IL-6	21865-1-AP, Proteintech, Wuhan, China
Anti-p50	ab32360, Abcam, Cambridge, UK
Anti-p65	ab16502, Abcam, Cambridge, UK
Anti-Lamin B1	12987-1-AP, Proteintech, Wuhan, China
Anti-ERK	16443-1-AP, Proteintech, Wuhan, China
Anti-p-ERK1/2	ab76299, Abcam, Cambridge, UK
Anti-p38	14064-1-AP, Proteintech, Wuhan, China
Anti-p-p38 (T180+Y182)	ab4822, Abcam, Cambridge, UK
Anti-NLRP3	NBP2-12446, Novus, Littleton, CO, USA
Anti-Smad3	25494-1-AP, Proteintech, Wuhan, China
Anti-p-Smad2 (ser465/ser467)	#18338, Cell Signaling Technology, Danvers, MA, USA
Anti-p-Smad3 (ser423/ser425)	#9520, Cell Signaling Technology, Danvers, MA, USA
Anti-caspase-1	ab179515, Abcam, Cambridge, UK
Anti-monocyte chemoattractant protein-1 (MCP-1)	66272-1-lg, Proteintech, Wuhan, China
Anti-Smad2	12570-1-AP, Proteintech, Wuhan, China
Anti-apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC)	sc-514414, Santa Cruz, Dallas, Texas, USA
Anti-IL-1β	WL00891, Wanleibio, Shenyang, China
Anti-IL-18	10663-1-AP, Proteintech, Wuhan, China
Anti-tumor necrosis factor-α (TNF-α)	60291-1-Ig, Proteintech, Wuhan, China
Anti-β-actin	sc-47778, Santa Cruz, Dallas, Texas, USA