Oxidative Medicine and Cellular Longevity / 2020 / Article / Fig 5

Research Article

Tribulus terrestris Ameliorates Oxidative Stress-Induced ARPE-19 Cell Injury through the PI3K/Akt-Nrf2 Signaling Pathway

Figure 5

Effects of EE-TT on mRNA expression levels of rodex-related genes in H2O2-treated ARPE-19 cells. ARPE-19 cells were pretreated with or without 1 mM H2O2 for 24 h, followed by a 24 h exposure to ethanol extracts of Tribulus terrestris (EE-TT). The mRNA expression levels of Nrf2 (a), CAT (b), SOD1 (c), SOD2 (d), GST-pi (e), HO-1 (f), NQO1 (g), and GCLM (h) for each sample were measured by real-time PCR; the fold changes of each gene are shown in the bar graph compared with the control sample (nontreated cells, value of 1). The data are shown as , , , vs. the control sample (non-treated cells); #, ##, ### vs. the sample with H2O2 only treated sample.
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