Gender Difference on the Effect of Omega-3 Polyunsaturated Fatty Acids on Acetaminophen-Induced Acute Liver Failure
GSK3β contributes to the β-catenin signaling activation in response to n-3 PUFAs. (a) APAP (400 mg/kg) was intraperitoneally injected to male or female WT and fat-1 transgenic mice (). The phosphorylated levels of β-catenin and GSK3β were determined by immunoblotting assay. (b–d) HepaRG cells were pretreated with 50 μM DHA for 2 hours or 100 nM E2 for overnight before stimulated with 20 mM APAP. (b) The phosphorylation of β-catenin and GSK3β was determined by immunoblotting analysis at the indicated time point after APAP stimulation. (c, d) After being stimulated with APAP for 24 hours, the association of β-catenin with GSK3β in the cells was evaluated by immunoprecipitation (c) and immunofluorescence staining (d). The data represent three independent experiments with similar results.