High-Fat Diet Aggravates Acute Pancreatitis via TLR4-Mediated Necroptosis and Inflammation in Rats

Figure 3

HFD exacerbated inflammatory response and necroptosis in the pancreas of AP rats. (a) TLR4 and RIP3 levels of pancreas were analyzed by Western blot. β-Actin served as the loading control. (b) The relative protein expression of TLR4 was expressed as fold changes compared with the control in the blot after normalized to β-actin. (c) The relative protein expression of RIP3 was expressed as fold changes compared with the control in the blot after normalized to β-actin. (d) Immunohistochemistry of NF-κB p65 in the pancreas. (e) The expression of NF-κB p65-positive nuclei in the pancreas. (f) Immunohistochemistry of TNF-α in the pancreas. (g) The mean integrated optical density (IOD) of TNF-α in the pancreas. versus prior subgroup, ^# versus SCD group at the same time point. Scale .

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