Ganoderic Acid D Protects Human Amniotic Mesenchymal Stem Cells against Oxidative Stress-Induced Senescence through the PERK/NRF2 Signaling Pathway

Table 1

Primer sequence of target genes.

Gene	Sequence (5→3)	GenBank ID	Length of product (bp)
14-3-3ζ	For: CCTGCATGAAGTCTGTAACTGAG; Rev: GACCTACGGGCTCCTACAACA	NM_001135702.1	100
PRDX3	For: ACAGCCGTTGTCAATGGAGAG; Rev: ACGTCGTGAAATTCGTTAGCTT	NM_006793.4	152
SIRT1	For: TAGCCTTGTCAGATAAGGAAGGA; Rev: ACAGCTTCACAGTCAACTTTGT	NM_001142498.1	160
SIRT2	For: CACGCAGAACATAGATACCCTG; Rev: CAGTGTGATGTGTAGAAGGTGC	NM_001193286.1	162
SIRT3	For: GACATTCGGGCTGACGTGAT; Rev: ACCACATGCAGCAAGAACCTC	NM_001017524.2	122
SIRT4	For: GCTTTGCGTTGACTTTCAGGT; Rev: CCAATGGAGGCTTTCGAGCA	NM_012240.2	79
SIRT5	For: GCCATAGCCGAGTGTGAGAC; Rev: CAACTCCACAAGAGGTACATCG	NM_001193267.2	157
SIRT6	For: CCCACGGAGTCTGGACCAT; Rev: CTCTGCCAGTTTGTCCCTG	NM_001193285.2	194
SIRT7	For: ACGCCAAATACTTGGTCGTCT; Rev: AGCACTAACGCTTCTCCCTTT	NM_016538.2	119
NRF2	For: TGGGAGTAGTTGGCAGAT; Rev: AGCGACGGAAAGAGTATGA	NM_006164.5	190
β-Catenin	For: GGGATGGTGGGTGTAAGA; Rev: GCTGGTGACAGGGAAGAC	NM_001904.4	148
ERK1	For: CTACACGCAGTTGCAGTACAT; Rev: CAGCAGGATCTGGATCTCCC	NM_002746.2	109
ERK2	For: TCTGGAGCAGTATTACGACCC; Rev: CTGGCTGGAATCTAGCAGTCT	NM_138957.3	134
β-Actin	For: TGGCACCCAGCACAATGAA; Rev: CTAAGTCATAGTCCGCCTAGAAGCA	NM_001101.3	186