Chaperone-Mediated Autophagy Markers LAMP2A and HSC70 Are Independent Adverse Prognostic Markers in Primary Resected Squamous Cell Carcinomas of the Lung

Figure 2

Validation of HSC70 immunohistochemical staining. (a, b) HSC70 knockdown in NB4 APL cells. (a) HSC70 knockdown efficiency of three independent shRNAs (shHSC70_1-3) was determined by western blotting and comparison to scramble shRNA transduced control cells (SHC002). (b) The most efficient HSC70 knockdown (shHSC70_3) was selected and subjected to immunohistochemistry. (c, d) 293 T cells were transiently transfected with an empty vector (ctrl) and HSC70 expression plasmid. (c) HSC70 expression was validated by western blotting. (d) 293 T cells were subjected to HSC70 immunohistochemistry.

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