Improvement of Endothelial Dysfunction of Berberine in Atherosclerotic Mice and Mechanism Exploring through TMT-Based Proteomics

Figure 7

Ingenuity Pathway Analysis (IPA) and western blotting validation of common differentially expressed proteins. (a) Top 15 canonical pathways according to IPA. The blue bars represent , and the orange points denote the ratio of differentially expressed proteins. (b–d) Top 3 networks generated by IPA. Network 1 is involved in metabolic disease, developmental disorder, and hereditary disorder (b); Network 2 is involved in energy production, lipid metabolism, and small molecule biochemistry (c); Network 3 is involved in lipid metabolism, small molecule biochemistry, and vitamin and mineral metabolism (d). (e) Validation of differentially expressed proteins PON1, GPD2, and APOA1 by western blotting. Data are shown as , . ^# versus WT and versus ApoE^-/-. WT: wild-type; BBR: berberine; PON1: paraoxonase 1; APOA1: apolipoprotein A I; GPD2: glycerol-3-phospate dehydrogenase 2.

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