Metformin Reduces the Senescence of Renal Tubular Epithelial Cells in Diabetic Nephropathy via the MBNL1/miR-130a-3p/STAT3 Pathway

Figure 2

MBNL1 was downregulated in senescent HK-2 cells, and metformin reduced cell senescence in HK-2 cells by upregulating MBNL1. (a) qRT-PCR was performed to detect expression levels of MBNL1 in HK-2 cells that were cultured in NG and HG for 72 h. (b) MBNL1 protein expression levels in HK-2 cells that were cultured in NG and HG for 72 h. The data are expressed as the (). , vs. the NG group; , vs. the NG group. (c) The expressions of MBNL1, miR-130a-3p, and STAT3 were detected in HK-2 cells with MBNL1 overexpression or inhibition by qRT-PCR. (d) The protein expression levels of MBNL1, STAT3, and P21 were detected to determine the effect of MBNL1 on senescence in HK-2 cells. The data are expressed as the (). , vs. the MBNL1(+)-NC group; , vs. the MBNL1(+)-NC group; , vs. the MBNL1(-)-NC group; , vs. MBNL1(-)-NC group. (e) The expressions of MBNL1, miR-130a-3p, and STAT3 were detected in HK-2 cells treated with metformin by qRT-PCR. (f) The protein expression levels of MBNL1, STAT3, and P21 were detected in HK-2 cells that were treated with metformin. The data are expressed as the (). , vs. the control group; , vs. the control group; , vs. the Met group; , vs. the Met group.

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