Metformin Reduces the Senescence of Renal Tubular Epithelial Cells in Diabetic Nephropathy via the MBNL1/miR-130a-3p/STAT3 Pathway

Figure 3

miR-130a-3p was downregulated in senescent HK-2 cells, and metformin reduced cell senescence in HK-2 cells by upregulating miR-130a-3p. (a) qRT-PCR was used to detect expression levels of miR-130a-3p in HK-2 cells that were cultured in NG and HG for 72 h. The data are expressed as the (). , vs. NG group. (b) The RNA expression levels of miR-130a-3p and STAT3 were detected in HK-2 cells after miR-130a-3p overexpression or knockdown. (c) The protein expression levels of STAT3 and P21 were detected in HK-2 cells. The data are expressed as the (). , vs. the miR-130a-3p(+)-NC group; , vs. the miR-130a-3p(-)-NC group; , vs. the miR-130a-3p(-)-NC group. (d) The RNA expression levels of miR-130a-3p and STAT3 were detected in HK-2 cells after treatment with metformin. (e) The protein expression levels of STAT3 and P21 were detected in HK-2 cells after treatment with metformin. The data are expressed as the (). , vs. the control group; , vs. the control group; , vs. the Met group; , vs. the Met group.

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