Metformin Reduces the Senescence of Renal Tubular Epithelial Cells in Diabetic Nephropathy via the MBNL1/miR-130a-3p/STAT3 Pathway

Figure 4

MBNL1 bound with miR-130a-3p, and metformin reduced cell senescence in HK-2 cells by upregulating MBNL1/miR-130a-3p signaling. (a) miR-130a-3p was identified in the MBNL1 complex. miR-130a-3p enrichment was measured using qRT-PCR. The data are expressed as the (). , vs. the anti-IgG group; , vs. the anti-IgG group; , vs. the NG group. (b) The levels of MBNL1 and GAPDH proteins that immunoprecipitated with miR-130a-3p were evaluated by Western blot. The expression levels of MBNL1 and GAPDH proteins are shown. (c) The Cell-Light EU Apollo567 RNA Stain Kit was used to label and capture newly synthesized RNA. (d) Relative levels of miR-130a-3p at different actinomycin D treatment times in the control group, MBNL1(+)-NC group, and MBNL1(+) group. (e) The expression levels of STAT3 and P21 were detected to determine the effect of MBNL1 and miR-130a-3p on senescence in HK-2 cells. The data are expressed as the (). , vs. the control group; , vs. the control group; , vs. the MBNL1(+) group; , vs. the MBNL1(+) group; , vs. the MBNL1(-) group; , vs. the MBNL1(-) group; , vs. the miR-130a-3p(+) group; , vs. the miR-130a-3p(+) group; , vs. the miR-130a-3p(-) group. (f) STAT3 and P21 protein expression levels were detected in HK-2 cells after treatment with metformin. The data are expressed as the (). , vs. the control group; , vs. the Met group; , vs. the Met group.

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