Metformin Reduces the Senescence of Renal Tubular Epithelial Cells in Diabetic Nephropathy via the MBNL1/miR-130a-3p/STAT3 Pathway

Figure 6

Metformin improved the renal function and pathological changes in db/db mice. (a) Western blot was used to determine the expressions of P-AMPK and AMPK in HK-2 cells that were treated with HG and HG+Met for 72 h. The data are expressed as the (). , vs. the HG group; , vs. the HG group. (b, c) The expressions of MBNL1 and miR-130a-3p were detected in HK-2 cells that were cultured in HG, HG+A-769662, and HG+Dorsomorphin for 72 h. (d) Fasting blood glucose was recorded every week beginning at 8 weeks of age. The data are expressed as the (). , vs. the db/m group at 8 weeks; , vs. the db/db group at 8 weeks; , vs. the db/m group at 16 weeks; , vs. the db/db group at 16 weeks; , vs. the db/m group at 24 weeks; , vs. the db/db group at 24 weeks. (e) Serum creatinine and (f) urine ACR were detected at different weeks. The data are expressed as the (). , vs. the db/m group at 16 weeks; , vs. the db/db group at 16 weeks; , vs. the db/m group at 24 weeks; , vs. the db/db group at 24 weeks. (g) HE staining, (h) PAS staining, and (i) Masson trichrome staining were performed in renal cortex sections from the db/m group, db/db group, and db/db+Met group at different weeks. .

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