The Inhibition of miR-873 Provides Therapeutic Benefit in a Lipopolysaccharide-Induced Neuroinflammatory Model of Parkinson’s Disease

Figure 5

The effects of miR-873 on autophagy in SH-SY5Y cells. The fluorescence of pEGFP-LC3 (green) was reduced in the cytoplasm of the cells (the nucleus was stained blue) transfected with miR-873 or sh-ABCA1 (a). The allosteric inhibitor of mTORC1, rapamycin, restored the miR-873-induced reduction in autophagic flux. The LC3II/LC3I protein expression ratio was reduced in the cells transfected with miR-873 or sh-ABCA1; moreover, the p62 protein accumulated in these cells (b). The data are expressed as the ; , , , and compared with the controls.

(a)

(b)