Anti-Influenza Activity of an Ethyl Acetate Fraction of a Rhus verniciflua Ethanol Extract by Neuraminidase Inhibition

Figure 3

Determination of cytotoxicity and antiviral activity of R. verniciflua Stokes (RVS) ethanol extract in MDCK cells. Viability of MDCK cells was assessed using MTS assay after treatment with indicated concentrations of RVS (0–400 μg/mL) for 48 h (a). Antiviral activities of RVS on influenza A/PR/8/34-GFP virus in MDCK cells. MDCK cells were treated with RVS (12.5, 25, 50, and 100 μg/mL) before influenza A virus (A/PR/8/34-GFP) infection, and cells were incubated with medium alone (CON) or 12.5, 25, 50, and 100 μg/mL of RVS before infection with A/PR/8/34-GFP () (b). GFP expression and reduction in viral replication using flow cytometry were assessed 24 h after viral infection in GHE-treated MDCK cells (c). Bar graph () statistics were determined with data from three experiments using one-way ANOVA with Tukey’s post hoc test, ; . n.s.: not significant, compared with the (RVSE untreated) samples.

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