Anti-Influenza Activity of an Ethyl Acetate Fraction of a Rhus verniciflua Ethanol Extract by Neuraminidase Inhibition

Figure 7

Determination of neuraminidase (NA) inhibition efficacy of the 10 components identified from RVSE. (a) Structures of 10 components of RVSE: 1, butin; 2, eriodictyol; 3, liquiritigenin; 4, naringenin; 5, 5-deoxyluteolin; 6, fisetin; 7, sulfuretin; 8, quercetin; 9, garbanzol; and 10, aromadendrin. (b) Measurement of the antiviral activity of RVSE components (10 μM) using NA inhibition assay. The influenza A virus A/PR/8/34 was added to the indicated concentrations of RVSE components. Fluorescence was measured using fluorescence spectrophotometry (excitation, 365 nm; emission, 415–445 nm). The treatment with components (c) 5 and (d) 7 demonstrated the highest NA inhibitory activity against all viruses. Bar graph () statistics were determined by three experiments’ data using one-way ANOVA with Tukey’s post hoc test, ; , compared with CON (untreated) preparations.

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