Olaquindox-Induced Liver Damage Involved the Crosstalk of Oxidative Stress and p53 In Vivo and In Vitro

Figure 5

p53 knockout or knockdown attenuated OLA-induced apoptosis in HCT116 and L02 cells. (a) Cell viability of HCT116 cells was estimated by MTT assays. (b) HCT116 cells were stained with Hoechst 33342 and observed under an inverted fluorescence microscopy (400x). (c) The histogram depicted the mean apoptotic rate of HCT116 cells. (d) HCT116 cells were stained with Annexin V-FITC/PI and detected by flow cytometry. (e) Cell viability of L02 cells was estimated by MTT assays. (f) L02 cells were stained with Annexin V-FITC/PI and detected by flow cytometry. (g) The histogram depicted the mean apoptotic rate of L02. The results represent the of three independent experiments. and , compared with the control; ^# and ^##, compared with the p53 normal group.

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