Olaquindox-Induced Liver Damage Involved the Crosstalk of Oxidative Stress and p53 In Vivo and In Vitro

Figure 7

Ablation of p53 attenuated OLA-induced mitochondrial dysfunction in vitro. (a) Cells were stained by Rhodamine 123 (10 μg/mL) and observed under a fluorescence microscope (400x). (b) Expressions of p53 Bax, Bcl-2, Cyt C caspase 9, and PARP in HCT116 cells were detected by western blotting analysis. (c) The densitometry analysis of p53, Bax/Bcl-2, Cyt C, caspase 9, and PARP by the ImageJ 1.46 software. (d) Expressions of p53 Bax, Bcl-2, caspase 9, caspase 3, and PARP in L02 cells were detected by western blotting analysis. (e) The densitometry analysis via the ImageJ 1.46 software. The results represent the of three independent experiments. and , compared with the control; ^# and ^##, compared with the p53 normal groups.

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