Olaquindox-Induced Liver Damage Involved the Crosstalk of Oxidative Stress and p53 In Vivo and In Vitro

Figure 8

Loss of p53 partially inhibited JNK/p38 pathway and activated autophagy pathway. (a) Western blot analysis for JNK, p38, LC3, and Beclin1 in HCT116 cells. (b) The densitometric analysis of the bands in HCT116 cells were performed via the ImageJ 1.46 software. (c) Western blot analysis for JNK, p38, LC3, and Beclin1 in L02 cells. (d) The densitometric analyses of the bands in L02 cells were performed by the ImageJ 1.46 software. (e) Fluorescence microscopy was used to observe the distribution of autophagosomes in L02 cells after MDC staining (400x). The results represent the of three independent experiments. and , compared with the control; ^# and ^##, compared with p53 normal groups.

(a)

(b)

(c)

(d)

(e)