Ophiopogonin D Increases SERCA2a Interaction with Phospholamban by Promoting CYP2J3 Upregulation

Figure 2

Effect of OPD on the expression of CYP2J3 in H9c2 cells and 293 T cells. (a) H9c2s were treated with different concentrations of OPD (0.25, 0.5, 1 μmol/L) for 24 h. CYP2J3 mRNA and protein expression were increased in OPD-treatment groups , versus the control group; versus the ISO-treatment group (1 μmol/L). (b) H9c2s were cotreated with ISO (1 μmol/L) and different concentrations of OPD (0.25, 0.5, 1 μmol/L) for 24 h. CYP2J3 mRNA and protein expression were decreased in the ISO-treatment group ^### versus the control group; , , and versus the ISO-treatment group. (c) CYP2J3-siRNA and negative control- (NC-) siRNA were transfected into H9c2s. Cells were treated with OPD (1 μmol/L) and ISO (1 μmol/L) as mentioned above. CYP2J3 mRNA and protein expression were decreased in the Si-CYP2J3 group, ^a versus the si-control group; the CYP2J3 protein and mRNA level were increased in the OPD-treated group, ^b versus the OPD-treated NC group. (d) CYP2J3-siRNA and NC-siRNA were transfected with H9c2s. Cells were cotreated with OPD (1 μmol/L) and ISO (1 μmol/L) as mentioned above. CYP2J3 mRNA and protein expression were decreased in the si-CYP2J3 group, ^c versus the si-control group; CYP2J3 mRNA and the protein expression were decreased in the ISO-treated si-CYP2J3 group, ^d versus the ISO-treated NC group. (e) 293 T cells were transfected with pLVX-IRES-ZsGreen-1-CYP2J3 plasmid for 24 h. Cells were treated with OPD (1 μmol/L) for 24 h. The CYP2J3 expression was increased in the OPD-treated+CYP2J3 transfected group versus the CYP2J3 transfected group, per group.

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