Ophiopogonin D Increases SERCA2a Interaction with Phospholamban by Promoting CYP2J3 Upregulation

Figure 3

OPD induces SERCA2a colocalization of SERCA2a and p-PLB in HF cells. Images were collected on the LSM880 confocal microscope with Plan-Apochromat ×63. (a) Images showing immunofluorescence of SERCA2a (red) and PLB (green) proteins in H9c2 cells and changes of their colocalization pattern following OPD (1 μmol/L) and ISO (1 μmol/L) administration. An embedded scattergram estimated the amount of each detected antigen based on colocalization. Colocalized pixels of yellow color are located along the diagonal of the scattergram. Compared with the control group, the green (PLB) intensity increased in the ISO-treated group while the red (SERCA2a) intensity decreased. The OPD-treated group showed decreased PLB intensity compared with the ISO-treated group.(b) Images showing immunofluorescence of SERCA2a (green) and p-PLB (red) proteins in H9c2 cells and changes of their colocalization pattern following OPD (1 μmol/L) and ISO (1 μmol/L) administration. An embedded scattergram estimated the amount of each detected antigen based on colocalization. Colocalized pixels of yellow color are located along the diagonal of the scattergram. Compared with the control group, the red (p-PLB) intensity decreased in the ISO-treated group. The OPD-treated group showed increased p-PLB intensity compared with the ISO-treated group.

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