Ophiopogonin D Increases SERCA2a Interaction with Phospholamban by Promoting CYP2J3 Upregulation

Figure 5

SERCA2a co-IP analysis of p-PLB and PLB proteins. (a) H9c2 cells were pretreated with ISO (1 μmol/L) and OPD (0.25, 0.5, 1 μmol/L) at the indicated concentrations mentioned above. The physical association between SERCA2a and PLB/p-PLB was examined by a co-IP assay. Compared with the ISO-treated group, OPD induced the interaction between SERCA2a and p-PLB in a dose-dependent manner. (b) CYP2J3-siRNA and NC-siRNA were transfected into H9c2s. Cells were cotreated with OPD (1 μmol/L) and ISO (1 μmol/L) as mentioned above. Western blot analysis of the interaction between SERCA2a and p-PLB/PLB proteins by co-IP. CYP2J3 depletion weakened the physical binding of SERCA2a and p-PLB in H9c2 cells, and OPD treatment improved the faint binding caused by CYP2J3.

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