Ophiopogonin D Increases SERCA2a Interaction with Phospholamban by Promoting CYP2J3 Upregulation

Figure 9

Effects of OPD on the phosphorylated status of PLB in ISO or Ang-II induced HF cells. (a) H-89 selectively inhibited Ser¹⁶ phosphorylation of PLB during AngII and OPD cotreatment. (b) KN-93 selectively inhibited Thr¹⁷ phosphorylation of PLB during ISO (1 μmol/L) and OPD (1 μmol/L) cotreatment. (c) PKA was phosphorylated in Ang-II induced HF cells, versus the control group. OPD promoted the expression of p-PKA (the activation state of PKA), ^# versus the Ang-II-treated group. Furthermore, there was no significance in the expression of CaMKII and p-CaMKII. (d) CaMKII was phosphorylated in ISO-induced HF cells, ^### versus the control group. OPD promoted the expression of p-CaMKII (activation state of CaMKII), ⁺⁺, ⁺⁺⁺ versus the ISO-treated group, per group. Furthermore, there was no significance in the expression of PKA and p-PKA.

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