What Is the Impact of Depletion of Immunoregulatory Genes on Wound Healing? A Systematic Review of Preclinical Evidence

Figure 2

A schematic diagram showing the influence of different immunoregulatory genes of knockout mice on the wound healing process, the main phases affected by genetic silencing, and consequently the main mechanisms involved in this process, and the most common techniques used for this type of analysis. KO: Knockout; ELISA: Enzyme-Linked Immunosorbent Assay; RT-PCR: Reverse Transcription Polymerase Chain Reaction; qPCR: Real-Time quantitative Polymerase Chain Reaction; IHC: Immunohistochemistry; TGF-β1: Transforming Growth Factor beta 1; VEGF: Vascular Endothelial Growth Factor; TNF-α: Tumor Necrosis Factor-alpha; IL-6: Interleukin-6; IL-1β: Interleukin-1 beta; ep: epidermis; dm: dermis; inf: inflammatory molecules.