Activation of the Melanocortin-1 Receptor by NDP-MSH Attenuates Oxidative Stress and Neuronal Apoptosis through PI3K/Akt/Nrf2 Pathway after Intracerebral Hemorrhage in Mice
Table 1
Experimental groups and mortality rate.
Experimental groups
Neurological test Brain water content
ELISA
IF staining
WB
TUNEL
Exclusion
Mortality
Subtotal
Experiment 1
Sham
4
6
0
0
10
ICH (6 h, 12 h, 24 h, 72 h, and 7 d)
4
3
6 (13.95%)
43
Experiment 2
Sham
6
6
0
0
12
ICH + PBS
6
6
0
2 (14.29%)
14
ICH + NDP-MSH (1.5 μg/mouse)
6
6
0
2 (14.29%)
14
ICH + NDP-MSH (5 μg/mouse)
6
6
0
0
12
ICH + NDP-MSH (15 μg/mouse)
6
6
2
2 (12.5%)
16
Experiment 3
Sham
4
0
0
4
ICH + PBS
6
4
0
0
10
ICH + NDP-MSH
6
4
0
1 (9.09%)
11
ICH + NDP-MSH + Scr siRNA
6
6
0
0
12
ICH + NDP-MSH + Mc1r siRNA
6
6
4
1
4 (19.05%)
21
Experiment 4
ICH + NDP-MSH + DMSO
6
6
0
1 (7.69%)
13
ICH + NDP-MSH + LY294002
6
6
4
1
2 (10.53%)
19
Total
30
54
8
72
20
7
20 (9.48%)
211
ICH: intracerebral hemorrhage; PBS: phosphate-buffered saline; Mc1r: melanocortin-1 receptor; LY294002: PI3K specific inhibitor; siRNA: small interfering RNA.
